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Immunogold labelling revealed the presence of lipoxygenase (LOX) in different parts and types of anther cells of Gagea lutea. LOX was found in the cytoplasm and close to ER elements in epidermal and endothecial cells, and close to the cell walls of the latter. The positive immunoreaction to LOX was less intense in the middle layers and the loculus of the anther, where single immunogold particles were...
In the research effects of bergapten (5-MOP) and peucedanin, furanocoumarins isolated from fruits of Peucedanum tauricum Bieb. (Apiaceae), on apoptosis, necrosis and heat-shock proteins Hsp 27 and Hsp 72 expression in human carcinoma cells HeLa B (ECACC No 85060701) were preliminary studied. The purity of analysed compounds was confirmed by RP HPLC, EI-MS and 1D, 2D NMR. For apoptosis and necrosis...
Lipoxygenase was localized in the primary leaves of Phaseolus coccineus (L.), seedlings treated with 25 µM Cd and in control plants using the immunogold method. The enzyme was localized mainly in the peripheral parts of protoplast of control plant cells. It was found in the cell wall, along the ER elements, at plastid lamellae and inside the mitochondria. In Cd-treated seedlings the elements of parenchyma...
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