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A study was designed to obtain hapolid and double haploid (DH) plants from cucumber cultivars tolerant to Pseudoperonospora cubensis. The main goal was to identify RAPD molecular markers associated with downy mildew resistance. On average, 20% of embryos generated in two experiments, were converted to haploid plants. RAPD markers that differentiated susceptible and resistant H and DH plants were identified...
In vitro organogenesis in hypocotyl explants of the pepper cultivar ‘Bryza’ was induced on MS medium containing 5 mg/l 6-benzyloaminopurine (BAP) and 1 mg/l indole-3-acetic acid (IAA). The hypocotyl explants were then inoculated with Agrobacterium tumefaciens LBA4404(pBI121). After 2 days of culture the first cell divisions were observed in the epidermis and cortex. After 6-7 days, numerous adventitious...
Carotenoids play an essential role especially in human nutrition. Single or a few carotenoid genes have been identified for different organisms including plants. There are some informations in the literature about regulation of carotenoid biosynthesis. While analyzing the complexity of carotenoid metabolic pathway, it is clearly visible that our know ledge about genetic background of carotenoid changes...
Pepper (Capsicum annuum L.) is an important crop plant worldwide used as a spice and vegetable. Unlike other species of the Solanaceae family, pepper was a recalcitrant species with regard to its capacity to in vitro regeneration. In many cultivars of pepper, the regeneration was reported via organogenesis of shoot buds from cotyledons and hypocotyl explants, anther culture, somatic embryogenesis...
Embryological studies were undertaken to compare the normal development of cucumber endosperm and embryo with that observed after pollination with gamma-irradiated pollen (0.1 and 0.3 kGy). Delayed penetration of the pollen tube occurred at both irradiation doses. Endosperm and embryo development was also delayed, but was initiated within 6 days after pollination in 100% of embryo sacs at 0.1 kGy...
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