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INTRODUCTION: How can we make mouse studies more reproducible? The obvious answer is the standardization of experimental conditions, minimization of human interference, automation of behavioral tests and data analysis, and introduction of data analysis pipelines to automate the process. Eco‑HAB, a system for automated measurement of social preference and in-cohort sociability in mice, provides a solution...
The α‐Ca2+/calmodulin‐dependent protein kinase II (αCaMKII), a key regulator of the glutamatergic synapse, has been implicated in many psychiatric disorders characterized by social impairments. Here we tested whether autophosphorylation of αCaMKII at threonine 286, which prolongs the activity of the enzyme, affects social behaviors in mice. We observed that autophosphorylation‐deficient (αCaMKII‐T286A)...
INTRODUCTION: The reproducibility of behavioural tests has been improved by the introduction of a number of automated experimental systems. One of such systems is IntelliCage™, which allows for sophisticated experimental designs. Despite the improved reproducibility of experiments, reported results may be rendered irreproducible due to errors introduced by manual data analysis and not standardized...
INTRODUCTION: Tweety homolog1 (Ttyh1) is a presumed volume-regulated Cl-channel. It has been proposed to participate in the regulation of neuronal morphology. AIM(S): We aimed to examine dendritic arborization and spine morphology of pyramidal neurons following TTYH1 overexpression in organotypic hippocampal cultures. METHOD(S): Rat organotypic cultures were co-transfected with TTYH1‑GFP‑Synapsin...
Due to technological advances in electrophysiology, there is renewed interest in the analysis of local field potentials recorded at many sites simultaneously. In this paper the main problems related to the analysis of LFP are presented, and recent developments in the data analysis methods are reviewed. The focus of the paper is on reconstruction of current source density from extracellular recordings...
BACKGROUND AND AIMS: Matrix metalloproteinase 9 (MMP-9) is locally translated in dendrites in response to synaptic stimulation. Its enzymatic activity at the synapse is involved in the reorganization of spine architecture and was shown to regulate spine morphology in Fragile X syndrome (FXS) which is caused by the loss of mental retardation protein (FMRP). Application of MMP-9 on neurons in culture...
Dendritic spines are targets of excitatory synaptic inputs. The morphology of a spine is linked to its function. For example, a mature dendritic spine resembles a mushroom, with a wide spine head connected to the dendrite by much thinner neck. Several studies link dimensions of that mushroom to the functioning of the synapse, for example it has been shown that the amplitude of uncaging potential at...
AIMS: Fluoxetine, a selective serotonine reuptake inhibitor, is commonly used to treat psychiatric disorders. Available data show that fluoxetine has limited side effects and, more importantly, may improve patient’s cognitive abilities. However, little is known about the mechanisms by which fluoxetine affects learning, especially appetitively motivated one. Thus, in the present project we investigated...
An abundance of behavioral data can be obtained with the IntelliCage system. Basic analysis of the data can be performed with a tool provided with the system, however more advanced analyses have to be performed manually. One of disadvantages of manual analysis is its extreme unefficiency since it requires significant effort. Another disadvantage is that it is vulnerable to human errors. The analysis...
To test methods of local field potential (LFP) analysis we need realistic ground truth data which demands plausible models of neural activity and of physical properties of the setup, tissue, and the electrodes. To interpret the recordings we often reconstruct the Current Source Density (CSD) from the LFP. In this work we study the effect of realistic conductivity profiles and the slice geometry on...
Perseveration, defined as resistance to change in routine and repetitive behaviors, is one of the core symptoms of Autism Spectrum Disorders. It was proposed that an inability to break habits, experienced by autistic people, corresponds, in animal models, to impaired performance in the learning tasks that assess ability to change a response strategy to obtain reinforcement. However, the results of...
Most of recent studies of the role of cortical feedback in thalamocortical loop focused on its effect on thalamo-cortical relay (TCR) cells of the dorsal lateral geniculate nucleus (LGN). In a previous, physiological study we showed in cat visual system that cessation of cortical input decreased spontaneous activity of TCR cells and increased spontaneous firing of recurrent inhibitory interneurons...
Local field potentials (LFP), the low-frequency part of extracellular electric potential, reflect dendritic processing of synaptic inputs to neuronal populations. Today one can easily record simultaneous potentials from multiple contacts. Due to the nature of electric field each electrode may record activity of sources millimeters away which leads to significant correlations between signals and complicates...
Local field potentials (LFP), the low-frequency part of the extracellular electric potentials, reflect dynamics of the brain at the population level. Because of technological advances it is now feasible to record LFP at tens of locations simultaneously. The interpretation of these signals is complicated by the fact that the electric signals propagate in the tissue, and the signal recorded at each...
Local field potentials (LFP), low-frequency part of extracellular electric potentials, seem to reflect dendritic processing of incoming activity to neural populations. Long-range nature of electric field leads to correlations even between remote recordings showing sources from millimeters away which complicates analysis of LFP. To get more insight it is convenient whenever possible to look for current...
The vibrissal system of rodents has become one of the dominant models for investigating the mechanisms of sensory information processing. However, the mechanisms underlying integration of multiple whisker input is not well understood. To address this question, we recorded local fi eld potentials (LFP) from the barrel cortex in of anaesthetised rat. Recording points were distributed on the 4 × 22 grid...
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