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The metallo‐β‐lactamase VIM‐31 differs from VIM‐2 by only two Tyr224His and His252Arg substitutions. Located close to the active site, the Tyr224His substitution is also present in VIM‐1, VIM‐4, VIM‐7 and VIM‐12. The VIM‐31 variant was reported in 2012 from Enterobacter cloacae and kinetically characterized. It exhibits globally lower catalytic efficiencies than VIM‐2. In the present study, we report...
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