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Isolation and detection of tumor cells (TCs) from a large amount of biological samples have been a major challenge in clinical diagnosis. To tackle this problem, a three-dimensional micro incubator using deflecting membranes has been reported by our group previously. However, it requires a relatively complicated fabrication process, which involves bonding of multiple polydimethyl siloxane (PDMS) layers...
This study reports a new platform capable of assembling carbon nanotubes (CNTs) between electrodes to form CNT-based nano sensors based on the combination of optically-induced dielectrophoresis (ODEP) force and dielectrophoresis (DEP) force. Indium-tin-oxide (ITO) electrodes and amorphous silicon (a-Si) layer were patterned and then used to assemble CNTs. By utilizing moving light patterns, the CNTs...
Detection of fish pathogens is crucial for premium aquaculture species with a high economic value. The current study therefore presents a new microfluidic system for detection of aquaculture pathogens. Pathogen DNA was first purified by using specific nucleotide probes, followed by amplifying pathogen-specific DNA fragments by adopting the loop-mediated-isothermal-amplification (LAMP) process. Furthermore,...
Opticaldielectrophoresis(ODEP)has been explored experimentally with success in manipulating microscale objects in the last 5 years. However, not much theoretical analyses have been performed to understand its operating principles in depth and also determine its limitations as a tool to manipulate micro- and nano-scale objects. In this paper, we present our work on establishing an equivalent electrical...
Configurable assembly technique of a DNA origami tile on Micro Electromechanical Systems (MEMS) using a microfluidic device to realize a Nanosystem was proposed. In this newly proposed approach, multiple DNA origami tiles fabricated by DNA origami technique are utilized as a sub-micro-scale functional building block on which nano-materials such as nano-particles and SWCNTs are assembled by DNA hybridization...
This paper presents an integrated microfluidic system for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) by utilizing antibody-conjugated magnetic beads. By using immunomagnetic beads conjugated with specific antibodies and on-chip nucleic acid amplification, the developed microfluidic system can detect MRSA bacteria with a high sensitivity and specificity when compared with...
This study presents a new method for generating tunable and uniform magnetic alginate microbeads, which can be widely employed in the field of the bead-based bioassays such as sample pretreatment or immunoassays. The proposed microfluidic chip comprises pneumatically-driven vibrators to continuously spot alginate microdroplets in a thin oil layer. Continuously, the temporarily formed alginate microdroplets...
This study reports a cell culture platform consisting of 48 microbioreactors for high-throughput perfusion three-dimensional (3-D) cell culture-based assays. Its main characteristics are the capability of conducting multiplex backflow-free medium delivery, and the function for both efficient and high-throughput micro-scale 3-D cell culture construct loading. In addition, a micro-scale perfusion 3-D...
This current study presents a new miniature, integrated system capable of rapid extraction of genomic DNA (gDNA) from saliva samples. Three major components of the DNA extraction chip, including six symmetrical, normally-closed microvalves, a sample transport/mixing unit and a waste unit are integrated in this device. The microfluidic DNA extraction chip was made of CNC machining and PDMS casting...
Systematic evolution of ligands by exponential enrichment (SELEX) is a screening technique that involves a progressive selection of highly specific ligands by performing repeated rounds of partition and amplification from a large combinatorial nucleic acid library. The products of the selection are usually called aptamers, which are short single-stranded deoxyribonucleic acid (ssDNA) or ribonucleic...
This study presents an integrated microfluidic system capable of rapid diagnosis and subtyping of influenza virus. Several critical modules including a ribonucleic acid (RNA) extraction module, a reverse-transcription polymerase chain reaction (RT-PCR) module and an optical detection module were integrated into the microsystem. Magnetic beads conjugated with specific nucleotide probes were first incubated...
Mitochondria DNA (mtDNA) plays an important role in both aging-associated degenerative diseases and cancers. In this study, a new microfluidic system integrating functional components including a mtDNA extraction module, a polymerase chain reaction (PCR) module and a micro-capillary electrophoresis (MCE) module has been developed. A new approach to vertically integrate these three modules has been...
This paper reports an innovative approach for the manipulation of micro-particles utilizing optically-induced dielectrophoresis (ODEP), which was generated by a flexible polymer-based substrate for biomedical applications. The ODEP platform fabricated on a thin-film polymer-based substrate has been reported as a promising technique for particle/cell manipulation. Without using sophisticate and high-temperature...
This paper reports a technique for manipulation of carbon nanotubes (CNTs). A photoconductive layer capable of generating a non-uniform electric field at illuminated sites was used to optically induce dielectrophoretic forces for manipulating CNTs. The light patterns enabled a variety of flexible modes for CNT manipulation since it was possible to dynamically reconfigure the optical patterns. This...
This study presents a new method for generating tunable and uniform magnetic alginate microspheres, which can be widely employed in the field of the bead-based bio sample pretreatment and enrichment process during the clinical diagnoses. A microfluidic device comprising microchannels and controllable moving-wall structures was fabricated by using MEMS processes. The key feature of the controllable...
The paper presents a new immunomagnetic-bead-based microfluidic system integrated with an optical detection module for rapid diagnosis of influenza infection. By using the immunomagnetic beads conjugated with influenza-specific antibodies, the developed system can detect influenza viral particles with a high sensitivity and specificity while compared with large-scale systems. The viral particles were...
The current study presents an innovative magnetic bead-based 3-dimensional (3D) micro-incubator for rapid purification of tumor cells (TCs) by using micro-electro-mechanical-systems (MEMS) technologies. With the incorporation of magnetic beads, specific antibodies conjugated onto the surface of the magnetic beads can be used to specifically recognize the TCs in the clinical body fluids, followed by...
C-reactive protein (CRP) is commonly known as a clinical marker, which has been identified as an independent predictor for cardiovascular diseases such as heart attack and stroke. In this study, a new microfluidic system for automatic measurement of CRP is reported. The entire process including sample pre-treatment, incubation between target CRP and anti-CRP antibody, developing process and detection...
This paper reports a new microfluidic chip capable of measuring and counting CD3/CD4 T helper cells. A T-cell count was essential for diagnosis of immunodeficiency and lymphocytic diseases. To achieve the measurement and counting of T helper cells, a new microfluidic device comprising a micro incubation and a micro flow cytometer module was presented in this study for sample pretreatment, measuring,...
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