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Pseudorabies virus (PRV) has been widely used as a live trans‐synaptic tracer for mapping neuronal circuits. Systematically identifying mature PRV virion proteomes and defining co‐purified host proteins are necessary to fully understand the detailed mechanism underlying PRV transmission processes. Here, a PRV virion purification strategy based on sorting with flow cytometry is developed and the mature...
DOI: 10.1002/pmic.201900009
In article number 1900009, Fei‐Long Yu et al. provide a comprehensive proteomics analysis of mature extracellular and intracellular Pseudorabies virus (PRV) virions based on a virion purification strategy. PRV virions are isolated from infected culture cells through a flow cytometry sorting‐based method, and the extracellular and intracellular PRV proteomes are characterized...
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