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Ascorbate (Asc) is a major redox buffer of plant cells, whose antioxidant activity depends on the ratio with its one‐electron oxidation product monodehydroascorbate (MDHA). The cytoplasm contains millimolar concentrations of Asc and soluble enzymes that can regenerate Asc from MDHA or fully oxidized dehydroascorbate. Also, vacuoles contain Asc, but no soluble Asc‐regenerating enzymes.
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