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Diphosphoinositol‐5‐pentakisphosphate (5‐PP‐IP5), also known as inositol heptakisphosphate (5‐IP7), has been described as a high‐energy phosphate metabolite that participates in the regulation of multiple cellular processes through protein binding or serine pyrophosphorylation, a posttranslational modification involving a β‐phosphoryl transfer. In this study, utilizing an immobilized 5‐IP7 affinity...
Necroptosis is an important form of lytic cell death triggered by injury and infection, but whether mixed lineage kinase domain-like (MLKL) is sufficient to execute this pathway is unknown. In a genetic selection for human cell mutants defective for MLKL-dependent necroptosis, we identified mutations in IPMK and ITPK1, which encode inositol phosphate (IP) kinases that regulate the IP code of soluble...
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