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Objective We report a truly quantitative technology for PD-L1 expression in non-small cell lung cancer (NSCLC). In addition, we present a non-enzymatic technology that creates a cell suspension from fresh tumor tissue so that either fine-needle aspiration (FNA) or fresh tissue can be used in this assay. Methods Non-enzymatic tissue homogenization (IncellPREP; IncellDx, Menlo Park, California) was...
The primary cilium is required for Sonic hedgehog (Shh) signaling in vertebrates. In contrast to mutants affecting ciliary assembly, mutations in the intraflagellar transport complex A (IFT-A) paradoxically cause increased Shh signaling. We previously showed that the IFT-A complex, in addition to its canonical role in retrograde IFT, binds to the tubby-like protein, Tulp3, and recruits it to cilia...
Jubb A M, Sa S M, Ratti N, Strickland L A, Schmidt M, Callahan C A & Koeppen H (2012) Histopathology61, 340–349
Neuropilin‐2 expression in cancerAims: Neuropilin‐2 is a coreceptor for vascular endothelial growth factor family members. Blockade of neuropilin‐2 is able to suppress lymphogenous metastasis in preclinical models. The aim of this study was to validate a protocol for the evaluation...
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