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All‐enzyme hydrogel (AEH) particles with a hydrodynamic diameter of up to 120 nm were produced intracellularly with an Escherichia coli‐based in vivo system. The inCell‐AEH nanoparticles were generated from polycistronic vectors enabling simultaneous expression of two interacting enzymes, the Lactobacillus brevis alcohol dehydrogenase (ADH) and the Bacillus subtilis glucose‐1‐dehydrogenase (GDH),...
This paper is based on a current research project and describes methods which aim to contribute to the estimation of the energy consumption in the production of goods. Today, the energy a product requires during its operation is the object of many activities in research and development. However, the energy necessary for the production of goods is very often not analyzed in comparable depth. The energy...
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