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Circulating immunoglobulin (Ig)G antibodies against M2 muscarinic acetylcholine receptors (M2 mAChR) have been implicated in Chagas' disease (ChD) pathophysiology. These antibodies bind to and activate their target receptor, displaying agonist‐like activity through an unclear mechanism. This study tested the ability of serum anti‐M2 mAChR antibodies from chronic ChD patients to modulate M2 muscarinic...