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Mutations within transpeptidase domain of penicillin‐binding protein 2B of the strains of Streptococcus pneumoniae leads to resistance against β‐lactam antibiotics. To uncover the important residues responsible for sensitivity and resistance, the recently determined three dimensional structures of penicillin‐binding protein 2B of both wild‐type R6 (sensitive) and mutant 5204 (resistant) strains along...