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The acetate‐bound form of the type II copper was found in the X‐ray structure of the multicopper oxidase CueO crystallized in acetate buffer in addition to the conventional OH−‐bound form as the major resting form. The acetate ion was retained bound to the type II copper even after prolonged exposure of a CueO crystal to X‐ray radiation, which led to the stepwise reduction of the Cu centres. However,...