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Trastuzumab is widely used in human epidermal growth factor receptor 2 (HER2)‐positive gastric cancer (GC) therapy, but ubiquitous resistance limits its clinical application. In this study, we first showed that CD44 antigen is a significant predictor of overall survival for patients with HER2‐positive GC. Next, we found that CD44 could be co‐immunoprecipitated and co‐localized with HER2 on the membrane...