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The C12 specific oxidation of hydroxysteroids is an essential reaction required for the preparation of pharmaceutical ingredients like ursodeoxycholic acid (UDCA) and chenodeoxycholic acid (CDCA), which can be synthesized by Wolff‐Kishner reduction of the obtained 12‐oxo‐hydroxysteroids. 12α‐hydroxysteroid dehydrogenases (12α‐HSDHs) have been shown to perform this reaction with high yields, under...