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An efficient and reproducible protein recovery procedure from Escherichia coli is presented. Recombinant strains overexpressing recombinant α-amylase were cultured in shaken flasks containing small glass beads. Most of the cells underwent mechanical lysis and more than 90% of the α-amylase content and also of other non-recombinant enzymes were released into the culture fluid after 24 h. The degree...