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Abstract We present a rapid in vitro method to scan the repair of DNA double-strand breaks (DSBs). A DSB was introduced at the EcoRI site within the lacZ gene of the plasmid pUC18 and the plasmid was exposed to cellular extracts from a wild-type repair-competent (RAD) and a mutant (rad52��) strain of the yeast Saccharomyces cerevisiae. The fidelity of rejoining was determined by the expression of...