I. Gaivão

article

The Comet assay for detection of DNA damage in canine sperm

AF Pereira, P Borges, A Fontbonne, L Cardoso, more

Reproduction in Domestic Animals > 52 > 6 > 1149 - 1152

Sperm DNA integrity is a fundamental prerequisite in fertilization and embryo development. Among DNA integrity tests, the Comet assay is an accurate and sensitive test for the detection of sperm oxidative damage. The aim of this work was to evaluate sperm oxidative damage using the Comet assay and to study the correlation between Comet and routine assays for the evaluation of semen quality. Dogs were...

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Pb low doses induced genotoxicity in Lactuca sativa plants

S. Silva, P. Silva, H. Oliveira, I. Gaivão, more

Plant Physiology and Biochemistry > 2017 > 112 > C > 109-116

Soil and water contamination by lead (Pb) remains a topic of great concern, particularly regarding crop production. The admissible Pb values in irrigation water in several countries range from ≈0.1 to ≈5 mg L⁻¹. In order to evaluate putative effects of Pb within legal doses on crops growth, we exposed Lactuca sativa seeds and seedlings to increasing doses of Pb(NO₃)₂ up to 20 mg L⁻¹. The OECD parameter...

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Are DNA-damaging effects induced by herbicide formulations (Roundup® and Garlon®) in fish transient and reversible upon cessation of exposure?

S. Guilherme, M.A. Santos, I. Gaivão, M. Pacheco

Aquatic Toxicology > 2014 > 155 > Complete > 213-221

Owing to the seasonality of crop cultivation and subsequent periodic/seasonal application of herbicides, their input to the aquatic systems is typically intermittent. Consequently, exposure of fish to this type of contaminants can be short and followed by a period of permanence in non-contaminated areas. Thus, the assessment of genotoxic endpoints in fish after removal of the contamination source...

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DNA and chromosomal damage induced in fish (Anguilla anguilla L.) by aminomethylphosphonic acid (AMPA)—the major environmental breakdown product of glyphosate

S. Guilherme, M. A. Santos, I. Gaivão, M. Pacheco

Environmental Science and Pollution Research > 2014 > 21 > 14 > 8730-8739

The assessment of the direct impact of breakdown products of pesticide components on aquatic wildlife is ecotoxicologically relevant, but frequently disregarded. In this context, the evaluation of the genotoxic hazard posed by aminomethylphosphonic acid (AMPA—the major natural degradation product of glyphosate) to fish emerges as a critical but unexplored issue. Hence, the main goal of the present...

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DNA damage in fish (Anguilla anguilla) exposed to a glyphosate-based herbicide – Elucidation of organ-specificity and the role of oxidative stress

S. Guilherme, I. Gaivão, M.A. Santos, M. Pacheco

Mutation Research - Genetic Toxicology and Environmental Mutagenesis > 2012 > 743 > 1-2 > 1-9

Organophosphate herbicides are among the most dangerous agrochemicals for the aquatic environment. In this context, Roundup ^® , a glyphosate-based herbicide, has been widely detected in natural water bodies, representing a potential threat to non-target organisms, namely fish. Thus, the main goal of the present study was to evaluate the genotoxic potential of Roundup ^® in the teleost...

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Differential genotoxicity of Roundup^® formulation and its constituents in blood cells of fish (Anguilla anguilla): considerations on chemical interactions and DNA damaging mechanisms

S. Guilherme, M. A. Santos, C. Barroso, I. Gaivão, more

Ecotoxicology > 2012 > 21 > 5 > 1381-1390

It has been widely recognized that pesticides represent a potential threat in aquatic ecosystems. However, the knowledge on the genotoxicity of pesticides to fish is still limited. Moreover, genotoxic studies have been almost exclusively focused on the active ingredients, whereas the effect of adjuvants is frequently ignored. Hence, the present study addressed the herbicide Roundup^®, evaluating the...

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Search results for: I. Gaivão

The Comet assay for detection of DNA damage in canine sperm

Pb low doses induced genotoxicity in Lactuca sativa plants

Are DNA-damaging effects induced by herbicide formulations (Roundup® and Garlon®) in fish transient and reversible upon cessation of exposure?

DNA and chromosomal damage induced in fish (Anguilla anguilla L.) by aminomethylphosphonic acid (AMPA)—the major environmental breakdown product of glyphosate

DNA damage in fish (Anguilla anguilla) exposed to a glyphosate-based herbicide – Elucidation of organ-specificity and the role of oxidative stress

Differential genotoxicity of Roundup^® formulation and its constituents in blood cells of fish (Anguilla anguilla): considerations on chemical interactions and DNA damaging mechanisms

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INFONA - science communication portal

Search results for: I. Gaivão

The Comet assay for detection of DNA damage in canine sperm

Pb low doses induced genotoxicity in Lactuca sativa plants

Are DNA-damaging effects induced by herbicide formulations (Roundup® and Garlon®) in fish transient and reversible upon cessation of exposure?

DNA and chromosomal damage induced in fish (Anguilla anguilla L.) by aminomethylphosphonic acid (AMPA)—the major environmental breakdown product of glyphosate

DNA damage in fish (Anguilla anguilla) exposed to a glyphosate-based herbicide – Elucidation of organ-specificity and the role of oxidative stress

Differential genotoxicity of Roundup® formulation and its constituents in blood cells of fish (Anguilla anguilla): considerations on chemical interactions and DNA damaging mechanisms

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Differential genotoxicity of Roundup^® formulation and its constituents in blood cells of fish (Anguilla anguilla): considerations on chemical interactions and DNA damaging mechanisms