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Background Coniophora olivacea is a basidiomycete fungus belonging to the order Boletales that produces brown-rot decay on dead wood of conifers. The Boletales order comprises a diverse group of species including saprotrophs and ectomycorrhizal fungi that show important differences in genome size. Results In this study we report the 39.07-megabase (Mb) draft genome assembly and annotation of C. olivacea...