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The effect of helium on cell survival during cryopreservation was studied using the HeLa and L929 cell lines. Cell suspensions were incubated in an atmosphere of helium, nitrogen, or air and frozen in the presence or absence of glycerol as a cryoprotectant. After thawing, the cell viability was evaluated by the Trypan Blue exclusion test and culture development for 18 h. Helium was found to provide...
Processes and thermodynamic parameters of dehydration of transition metal (Ni, Co, and Fe) sulfate heptahydrate crystals grown in aqueous solutions have been investigated by optical thermomicroscopy, X-ray analysis, and differential scanning calorimetry. Values of temperature and specific heat of the first stage of dehydration have been determined, as well as the conditions under which the process...
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