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This study aimed to establish an in vitro protocol for callus induction and cell suspension cultures of silver birch (Betula pendula Roth) and to assess the antioxidant activity of cell extract and the accumulation of betulin (B) and betulinic acid (BA). Callus was induced from the inner bark of stems on Gamborg (B5) and Nagata-Takebe (NT) media, and 23 different concentrations of growth regulators...