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This paper presents a microelectrothermofluidic packaging of integrated microdevice for viral ribonucleic acid (RNA) extraction from blood samples and its amplification through a reverse transcription (RT)-polymerase chain reaction (PCR) method for application to the point-of-care (POC) infectious disease diagnostics. Integrated microdevice consists of solid phase extraction microchannel structure,...
To meet the requirements of infectious diseases identification, a sealed and fully enclosed cartridge with self-contained reagents was developed. The inlet and outlet ports of the cartridge are self-sealing. The waste produced during the diagnostic process was collected in a waste bag, which is enclosed in the cartridge. Two different types of waste bags were designed to reduce the overall thickness...
This paper reports on the comparison analysis of four main types of silicon-based microfilter for isolation of white blood cells (WBCs) from red blood cells (RBCs) in a given whole blood. The microfilter designs, namely, weir, pillar, crossflow, and membrane, all impose the same cut-off size of 3.5 μm to selectively trap WBCs. Using human whole blood, the microfilters have been characterized and compared...
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