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The fluorescent probe techniques have been widely applied. When the fluorescence probes are selectively located in nano-structures, the fluorescence properties are highly influenced by the environment. Here, we systematically studied the location-influenced fluorescence of AIEgens in the microphase-separated structures. The AIEgen tetraphenylethene (TPE) was doped into polystyrene-b-polyisoprene (PS-{tib}-PI)...