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The electron transfer reactions involving QA and QB were investigated in Rb. capsulatus RCs where the QB site was mutated to contain 42 residues from the QA site. The RCs have M220–M261 in the QA site substituted for L193–L227 in the QB site plus the M subunit second-site mutations, M144MI and M145AS, which had been found to restore the ability of the bacteria to grow photosynthetically. These mutants...
Recently steady-state and picosecond time-resolved absorption and fluorescence spectroscopy on the Green Fluorescent Protein (GFP) have been interpreted by a mechanism where the key process is an excited state deprotonation of the chromophore (M. Chattoraij, B.A. King, G.U. Bublitz and S.G. Boxer, Proc. Natl. Acad. Sci. USA, 93 (1996) 8362-8367). Such a conclusion was borne out by the mirror image...
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