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Mammalian heart has been reported to express AC isozymes (types V and VI) that are inhibited by < μM [Ca 2+ ]; avian heart has been reported to express adenylyl cyclase activity that is inhibited by < μM [Ca 2+ ]. We have used reverse transcription polymerase chain reaction (RT-PCR) to determine that type V and VI AC mRNAs are present in freshly isolated ventricular...
When chick cardiac cells are maintained in serum-free NCTC-135 medium, the pH i dependence of the Na + / H + exchanger is much more acidic (pK = 7.0) than when a serum-free M199 medium is used (pK > 7.5). Phorbol esters activate the Na + /H + exchanger and produce a cell alkalinization in cells maintained in NCTC-135 medium by shifting the pH i dependence...
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