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The purpose of this study was to determine the effect of hypoxia on caspase-8 and -9 gene and protein expression and activity in corneal epithelium. Non-transformed human corneal epithelial cells (HCEC) were cultured in 2% oxygen. A cDNA expression array coupled with densitometric analysis was used to compare relative mRNA expression levels of 96 apoptosis-related genes in hypoxic and normoxic HCEC...
The objective of this study by the authors was to investigate the ultrastructural and physiological effects of exposure of the human corneal endothelium to mitomycin at concentrations of 20 ug/mL and 200 ug/mL using electron microscopy and in vitro specular perfusion techniques.
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