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Optical techniques such as two-photon (2p) calcium imaging have the potential to transform the way we interrogate neural circuits, both in the realm of basic neuroscience and in the development of brain-machine interfaces (BMIs). This may be possible by overcoming some of the limitations of electrophysiological methods. Here we ask if optical imaging signals, in particular 2p GCaMP6 calcium imaging...
A mechanistic understanding of neural computation requires determining how information is processed as it passes through neurons and across synapses. However, it has been challenging to measure membrane potential changes in axons and dendrites in vivo. We use in vivo, two-photon imaging of novel genetically encoded voltage indicators, as well as calcium imaging, to measure sensory stimulus-evoked...
Aspergillus niger is an effective secretor of glycoside hydrolases that facilitate the saprophytic lifestyle of the fungus by degrading plant cell wall polysaccharides. In the present study, a series of dynamic zymography assays were applied to quantify the secreted glycoside hydrolases of A. niger cultured in media containing different carbon sources. Differences in the diversity and concentrations...
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