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This report deals with the scanning acoustic microscope for imaging cross sectional characteristic acoustic impedance of biological soft tissues. A focused pulse wave is transmitted to the object placed on the ??rear surface?? of a plastic substrate. The reflected signals from the target and reference are interpreted into local acoustic impedance. Two-dimensional profile is obtained by scanning the...
SAM technology has been applied in medical and biological studies for 1) intra-operative pathological examinations since it does not require special staining techniques, 2) determining the origin of echo in clinical ultrasound imaging and 3) assessing the elastic properties of biological materials. In this short course, some examples of the application of scanning acoustic microscope (SAM) systems...
We are proposing the acoustic microscope for imaging cross sectional acoustic impedance of biological tissues. A focused acoustic beam is transmitted to the object placed on the ldquorear surfacerdquo of a plastic substrate. By scanning the focal point on the surface, a 2-D reflection intensity profile is obtained. A reference material is observed under the same condition. The reflection is interpreted...
Two-dimensional acoustic impedance imaging is useful for observation of living organs without invasion. In order to visualize the distribution of specific functional proteins in the cerebellar cortex, we proposed direct or complex-including heavy metal treatment to elevate the acoustic impedance of a particular cerebellar layer. Heavy metal binding was useful for acoustic impedance imaging; however,...
This report deals with the scanning acoustic microscope for imaging cross sectional acoustic impedance of biological soft tissues. A focused acoustic beam with a wide frequency range up to about 100 MHz was transmitted to the tissue object in contact with the "rear surface" of plastic substrate. The reflected signals from the target and reference are interpreted into local acoustic impedance...
Ultrasound speed and impedance microscopy was developed in order to develop in vivo imaging system. The sound speed mode realized non-contact high resolution imaging of cultured cells. This mode can be applied for assessment of biomechanics of the cells and thinly sliced tissues. The impedance mode visualized fine structures of the surface of the rat's brain. This mode can be applied for intra-operative...
Acoustic microscopy is expected to be a powerful tool for observing biological matters without chemical staining. We have proposed a new method for two-dimensional acoustic impedance imaging for biological tissue that can perform micro-scale observation without preparing a sliced specimen. A tissue was attached on a 0.5 mm-thick plastic substrate. An acoustic pulse was transmitted from the rear side...
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