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Soybean nodules contain four major leghemoglobin (Lb) components, Lba, Lbc1, Lbc2 and Lbc3. A sensitive and selective method for quantitative analysis of the four Lb components was devised with capillary isoelectric focusing (CIEF). The changes in the concentrations of four Lb components in nodules during the initial stages of development were compared between hypernodulating soybean mutant NOD1–3...
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