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Signalling of abscisic acid (ABA) in plants is characterized by an amazing number of secondary messengers that are part of the pathway or modulate the specific hormonal responses by interference with other signal transduction chains. In guard cells, a fast turgor-regulatory pathway triggered by ABA can be distinguished from a slower signalling pathway to the nucleus. The former is characterized by...
Signalling of abscisic acid (ABA) in plants is characterized by an amazing number of secondary messengers that are part of the pathway or modulate the specific hormonal responses by interference with other signal transduction chains. In guard cells, a fast turgor-regulatory pathway triggered by ABA can be distinguished from a slower signalling pathway to the nucleus. The former is characterized by...
Protein phosphatases 2C (PP2Cs) exhibit diverse regulatory functions in signalling pathways of animals, yeast and plants. ABI1 is a PP2C of Arabidopsis that exerts negative control on signalling of the phytohormone abscisic acid (ABA). Characterisation of the redox sensitivity of ABI1 revealed a strong enzymatic inactivation by hydrogen peroxide (H 2 O 2 ) which has recently been implicated...
Abstract. ABI1 and ABI2 are two protein serine/threonine phosphatases of type2C (EC3.1.3.16) that act as key regulators in the responses of Arabidopsis thaliana (L.) Heynh. to abscisic acid (ABA). They are involved in the control of ABA-mediated seed dormancy, stomatal closure and vegetative growth inhibition. Analysis of the enzymatic properties of ABI2 revealed high sensitivities towards protons...
The in vivo regulation of phosphoenolpyruvate carboxylase (PEPCase; EC 4.1.1.31), was studied in purified guard cell protoplasts (GCPs) of Vicia faba L. Incubation of GCPs with fusicoccin (FC) led to the rapid activation of PEPCase and reduced its sensitivity towards the feedback-inhibitor malate. This was accompanied by an increase in the phosphorylation state of the enzyme. Additionally, PEPCase...
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