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For the optical resolution of R-(−)-mandelic acid from (±)-mandelic acid, Pseudomonas sp. MA02, which assimilated S-(+)-mandelic acid as carbon and energy source, was isolated from soil. Using the fed-batch culture under optimal condition, R-(−)-mandelic acid was accumulated up to the maximum theoretical yield of 50% (30 g l−1) and entiomeric excess of 99.4%.
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