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Abstract An African (Kenyan) strain of Babesia bigemina, Muguga (B2-1), was inoculated into a calf from a stabilate and blood from the calf was used to establish the parasite in vitro. The strain has been cultured continuously for 20 months, initially in bovine erythrocytes with 60% adult bovine serum, later, with 50%. Cultures were incubated at 37C in RPMI 1640 medium with a gas mixture of 1% O2,...