Search results for: Waleed Ahmad Khattak

Items from 1 to 19 out of 19 results

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Current advancements of magnetic nanoparticles in adsorption and degradation of organic pollutants

Mazhar Ul-Islam, Muhammad Wajid Ullah, Shaukat Khan, Shehrish Manan, more

Environmental Science and Pollution Research > 2017 > 24 > 14 > 12713- 12722

Nanotechnology is a fast-emerging field and has received applications in almost every field of life. Exploration of new synthetic technologies for size and shape control of nanomaterials is getting immense consideration owing to their exceptional properties and applications. Magnetic nanoparticles (MNPs) are among the most important group of nanoparticles thanks to their diverse applications in medical,...

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Metabolic engineering of synthetic cell-free systems: Strategies and applications

Muhammad Wajid Ullah, Waleed Ahmad Khattak, Mazhar Ul-Islam, Shaukat Khan, more

Biochemical Engineering Journal > 2016 > 105 > Part B > 391-405

Recently, the focus of biotechnological research has shifted towards the improvement of biological processes. Among the various approaches to achieve this goal, the use of cell-free technology is receiving considerable attention because it offers several economic and technical benefits. This technology improves biological processes in several ways, leading to enhanced efficacy, stability, specificity,...

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Bacterial cellulose–poly(3,4-ethylenedioxythiophene)–poly(styrenesulfonate) composites for optoelectronic applications

Shaukat Khan, Mazhar Ul-Islam, Waleed Ahmad Khattak, Muhammad Wajid Ullah, more

Carbohydrate Polymers > 2015 > 127 > Complete > 86-93

Electrically conducting bacterial cellulose (BC) membranes were prepared by ex situ incorporation of poly(3,4-ethylenedioxythiophene)–poly(styrenesulfonate) (PEDOT:PSS) into BC pellicles. The BC pellicles were immersed into an aqueous solution of PEDOT:PSS for 6, 12, 18, or 24h, and the resultant composites were vacuum dried at ambient temperature. The structural features of the composites were determined...

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Production, Characterization and Physico-mechanical Properties of Bacterial Cellulose from Industrial Wastes

Waleed Ahmad Khattak, Taous Khan, Mazhar Ul-Islam, Fazli Wahid, more

Journal of Polymers and the Environment > 2015 > 23 > 1 > 45-53

In the current study, two different alternative media including black strap molasses (BSM) and molasses from condensation unit of brewery industry (MB) were evaluated for production of bacterial cellulose (BC) in comparison to chemically defined Hestrin–Schramm (HS) medium as a control under static and shaking conditions. In static culture, the BC production was 2.90, 1.74, 2.41 g/l for BSM, MB and...

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Production, characterization and biological features of bacterial cellulose from scum obtained during preparation of sugarcane jaggery (gur)

Waleed Ahmad Khattak, Taous Khan, Mazhar Ul-Islam, Muhammad Wajid Ullah, more

Journal of Food Science and Technology > 2015 > 52 > 12 > 8343-8349

Bacterial cellulose (BC) has been given an ample attention due to its high potential for many industrial applications. However, the high cost of production medium has hindered the commercialization of BC. Several efforts have been made to explore cheep, raw and waste sources for BC production. The current study aims at investigating the BC production from a waste source; the scum obtained during preparation...

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Enhanced bio-ethanol production via simultaneous saccharification and fermentation through a cell free enzyme system prepared by disintegration of waste of beer fermentation broth

Shaukat Khan, Mazhar Ul-Islam, Waleed Ahmad Khattak, Muhammad Wajid Ullah, more

Korean Journal of Chemical Engineering > 2015 > 32 > 4 > 694-701

Current study illustrates the effect of high yeast cell density contained in the waste of beer fermentation broth (WBFB) on bio-ethanol production through simultaneous saccharification and fermentation (SSF). WBFB was disintegrated (DW) and comparatively evaluated against nondisintegrated WBFB (NDW) for bio-ethanol production at variant temperatures. Final bio-ethanol levels of 36.38 g/L and...

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Bacterial cellulose-titanium dioxide nanocomposites: nanostructural characteristics, antibacterial mechanism, and biocompatibility

Shaukat Khan, Mazhar Ul-Islam, Waleed Ahmad Khattak, Muhammad Wajid Ullah, more

Cellulose > 2015 > 22 > 1 > 565-579

Regenerated bacterial cellulose (RBC) nanocomposites with titanium dioxide nanoparticles (TiO₂ NPs) were prepared with the aim to enhance the bactericidal activity and tissue regeneration properties of BC. Powdered BC was dissolved in an N-methylmorpholine-N-oxide/water (NMMO/H₂O) system, TiO₂ NPs were mixed into the BC solution, and RBC-TiO₂ nanocomposite films were prepared by casting the composite...

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Encapsulated yeast cell-free system: A strategy for cost-effective and sustainable production of bio-ethanol in consecutive batches

Muhammad Wajid Ullah, Waleed Ahmad Khattak, Mazhar Ul-Islam, Shaukat Khan, more

Biotechnology and Bioprocess Engineering > 2015 > 20 > 3 > 561-575

This study was intended to develop an encapsulated yeast cell-free system (EyCFS) by confining yeast cell-free lysate within a calcium alginate capsule. The system was evaluated for bio-ethanol production at elevated temperatures and was compared to a bare yeast cell-free system (ByCFS). Fermentation of 10 g/L glucose with shaking (150 rpm), using 2 mg/mL cell-free proteins in the ByCFS produced 3...

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Bio-ethanol production through simultaneous saccharification and fermentation using an encapsulated reconstituted cell-free enzyme system

Muhammad Wajid Ullah, Waleed Ahmad Khattak, Mazhar Ul-Islam, Shaukat Khan, more

Biochemical Engineering Journal > 2014 > 91 > Complete > 110-119

An encapsulated reconstituted cell-free enzyme system was developed through liquid-droplet forming method by using endogenous glycolytic and fermentation enzymes from yeast cells and exogenously added saccharification enzymes, cofactors, and ATPase. It was evaluated for bio-ethanol production through simultaneous saccharification and fermentation (SSF) at various temperatures, pH, and cell-free enzyme...

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Yeast cell-free enzyme system for bio-ethanol production at elevated temperatures

Waleed Ahmad Khattak, Mazhar Ul-Islam, Muhammad Wajid Ullah, Bowan Yu, more

Process Biochemistry > 2014 > 49 > 3 > 357-364

A yeast cell-free enzyme system containing an intact fermentation assembly and that is capable of bio-ethanol production at elevated temperatures in the absence of living cells was developed to address the limitations associated with conventional fermentation processes. The presence of both yeast glycolytic and fermentation enzymes in the system was verified by SDS-PAGE and LC–MS/MS Q-TOF analyses...

article

Developmental strategies and regulation of cell-free enzyme system for ethanol production: a molecular prospective

Waleed Ahmad Khattak, Muhammad Wajid Ullah, Mazhar Ul-Islam, Shaukat Khan, more

Applied Microbiology and Biotechnology > 2014 > 98 > 23 > 9561-9578

Most biomanufacturing systems developed for the production of biocommodities are based on whole-cell systems. However, with the advent of innovative technologies, the focus has shifted from whole-cell towards cell-free enzyme system. Since more than a century, researchers are using the cell-free extract containing the required enzymes and their respective cofactors in order to study the fundamental...

article

Synthesis of regenerated bacterial cellulose-zinc oxide nanocomposite films for biomedical applications

Mazhar Ul-Islam, Waleed Ahmad Khattak, Muhammad Wajid Ullah, Shaukat Khan, more

Cellulose > 2014 > 21 > 1 > 433-447

Regenerated bacterial cellulose (RBC) composites with zinc-oxide nanoparticles (ZnO) were prepared using a new strategy for enhanced biomedical applications of BC. Powdered BC was dissolved in N-methylmorpholine-N-oxide, and different concentrations of ZnO nanoparticles were mixed into the BC solution. RBC, RBC-ZnO1 (1 % ZnO) and ZnO-RBC2 (2 % ZnO) nanocomposite films were prepared by casting the...

article

Overview of bacterial cellulose composites: A multipurpose advanced material

Nasrullah Shah, Mazhar Ul-Islam, Waleed Ahmad Khattak, Joong Kon Park

Carbohydrate Polymers > 2013 > 98 > 2 > 1585-1598

Bacterial cellulose (BC) has received substantial interest owing to its unique structural features and impressive physico-mechanical properties. BC has a variety of applications in biomedical fields, including use as biomaterial for artificial skin, artificial blood vessels, vascular grafts, scaffolds for tissue engineering, and wound dressing. However, pristine BC lacks certain properties, which...

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Enhanced production of bioethanol from waste of beer fermentation broth at high temperature through consecutive batch strategy by simultaneous saccharification and fermentation

Waleed Ahmad Khattak, Taous Khan, Jung Hwan Ha, Mazhar Ul-Islam, more

Enzyme and Microbial Technology > 2013 > 53 > 5 > 322-330

Malt hydrolyzing enzymes and yeast glycolytic and fermentation enzymes in the waste from beer fermentation broth (WBFB) were identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). A new ‘one-pot consecutive batch strategy’ was developed for efficient bio-ethanol production by simultaneous saccharification and fermentation (SSF) using WBFB without additional enzymes, microbial...

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Effect of post-synthetic processing conditions on structural variations and applications of bacterial cellulose

Mazhar Ul-Islam, Waleed Ahmad Khattak, Minkyung Kang, Sang Min Kim, more

Cellulose > 2013 > 20 > 1 > 253-263

Physicochemical properties of materials can be amended by altering their physical structure through different processing conditions. The present study was conducted to investigate the post-synthesis structural variations and physico-mechanical properties of bacterial cellulose (BC) sheets prepared using different drying methods. Wet BC sheets of the same origin were freeze dried (BC-FD), dried at...

article

Challenges in the development of drugs for the treatment of tuberculosis

Adeeb Shehzad, Gauhar Rehman, Mazhar Ul-Islam, Waleed Ahmad Khattak, more

Brazilian Journal of Infectious Diseases > 2013 > 17 > 1 > 74-81

Tuberculosis infection is a serious human health threat and the early 21st century has seen a remarkable increase in global tuberculosis activity. The pathogen responsible for tuberculosis is Mycobacterium tuberculosis, which adopts diverse strategies in order to survive in a variety of host lesions. These survival mechanisms make the pathogen resistant to currently available drugs, a major contributing...

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Partial purification of saccharifying and cell wall-hydrolyzing enzymes from malt in waste from beer fermentation broth

Waleed Ahmad Khattak, Minkyung Kang, Mazhar Ul-Islam, Joong Kon Park

Bioprocess and Biosystems Engineering > 2013 > 36 > 6 > 737-747

A number of hydrolyzing enzymes that are secreted from malt during brewing, including cell wall-hydrolyzing, saccharide-hydrolyzing, protein-degrading, lipid-hydrolyzing, and polyphenol and thiol-hydrolyzing enzymes, are expected to exist in an active form in waste from beer fermentation broth (WBFB). In this study, the existence of these enzymes was confirmed by sodium dodecyl sulfate polyacrylamide...

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Bacterial cellulose-MMTs nanoreinforced composite films: novel wound dressing material with antibacterial properties

Mazhar Ul-Islam, Taous Khan, Waleed Ahmad Khattak, Joong Kon Park

Cellulose > 2013 > 20 > 2 > 589-596

This study was conducted to develop nanocomposite films of bacterial cellulose (BC) and montmorillonite (MMT) with potent antibacterial activity and potential therapeutic value in wound healing and tissue regeneration. Different composites were prepared through impregnation of BC sheets with 2 and 4 % suspensions of MMT, Na-MMT, Ca-MMT and Cu-MMT. These modified MMTs were prepared through cation exchange...

article

Prospects of reusable endogenous hydrolyzing enzymes in bioethanol production by simultaneous saccharification and fermentation

Waleed Ahmad Khattak, Mazhar Ul-Islam, Joong Kon Park

Korean Journal of Chemical Engineering > 2012 > 29 > 11 > 1467-1482

This study was conducted to evaluate the presence, origination and classification of various hydrolyzing enzymes from malt and their specified hydrolyzing effects on various substrates for bioethanol production and to link these characteristics with the future prospects of bioethanol production. These enzymes are categorized as cell wall, starch, protein, lipid, polyphenol and thiol hydrolyzing enzymes...

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