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Cefepime remains an important antibiotic for severe bacterial infections, yet some meta-analyses have shown elevated mortality among patients randomized to it. Therapeutic drug monitoring (TDM) of β-lactam antibiotics is increasing, but optimal plasma concentrations remain unknown. We examined clinical outcomes of patients undergoing cefepime TDM in an initial effort to define the drug's toxicity...
In order to increase the throughput of protein identification and characterisation in proteome studies, we investigated three methods of performing protein digestion in parallel. The first, which we term “One-Step Digestion-Transfer” (OSDT), is based on protein digestion during the transblotting process. It involves the use of membranes containing immobilised trypsin which are intercalated between...
Identification and characterization of all proteins expressed by a genome in biological samples represent major challenges in proteomics. Today’s commonly used high throughput approaches combine two-dimensional electrophoresis (2-DE) with peptide mass fingerprinting (PMF) analysis. Although automation is often possible, a number of limitations still adversely affect the rate of protein identification...
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