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The cDNA encoding cytochrome P-45017α from bovine adrenal cortex was expressed in Saccharomyces cerevisiae under the control of the galactose-inducible GAL10 promoter. Carbon monoxide difference spectra of the galactoseinduced yeast cells showed expression of about 240 nmol of P-45017α per liter of the culture. Binding of progesterone to the cytochrome P-45017α was clearly detectable already with...