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Borrelia and Bartonella DNA and tick-borne encephalitis virus (TBEV) RNA were detected in Ixodes persulcatus Schulze ticks collected in May 2009 in Chelyabinsk region, Ural, Russia by means of PCR with subsequent electrophoresis or real-time PCR with fluorescent hydrolysis probes. Borrelia garinii and Borrelia afzelii DNA were found in 36.6 ± 5.0% of taiga ticks with bacterial loads 101–106 DNA copies...