Search results for: Mariolina Gulli

Items from 1 to 19 out of 19 results

article

Heavy metals modulate DNA compaction and methylation at CpG sites in the metal hyperaccumulator Arabidopsis halleri

Serena Galati, Mariolina Gullì, Gianluigi Giannelli, Antonella Furini, more

Environmental and Molecular Mutagenesis > 62 > 2 > 133 - 142

Excess heavy metals affect plant physiology by inducing stress symptoms, however several species have evolved the ability to hyperaccumulate metals in above‐ground tissues without phytotoxic effects. In this study we assume that at subcellular level, different strategies were adopted by hyperaccumulator versus the non‐accumulator plant species to face the excess of heavy metals. At this purpose the...

article

Epigenetic modifications preserve the hyperaccumulator Noccaea caerulescens from Ni geno‐toxicity

Mariolina Gullì, Laura Marchi, Rosaria Fragni, Annamaria Buschini, more

Environmental and Molecular Mutagenesis > 59 > 6 > 464 - 475

The Ni hyperaccumulator Noccaea caerulescens has adapted to live in a naturally stressed environment, evolving a complex pattern of traits to cope with adverse conditions. Evidence is accumulating regarding the important role of epigenetic modifications in regulating plant responses to stress. In this study, we present data from the natural “open‐field” adaptation of the Ni hyperaccumulator N. caerulescens...

article

Detection of allergen coding sequences of kiwi, peach, and apple in processed food by qPCR

Sara Graziano, Mariolina Gullì, Nelson Marmiroli

Journal of the Science of Food and Agriculture > 98 > 8 > 3129 - 3139

BACKGROUND Food traceability becomes lifesaving for persons suffering severe allergy or intolerance, and therefore need a complete avoidance of the immune‐trigger food. This paper describes how to fingerprint the presence of some allergenic species (kiwi, peach, and apple) in foods by quantitative real‐time PCR (qPCR). RESULTS Five DNA extraction procedures were tested on fruits and foods. The results...

article

Development and validation of a SYBR‐Green I Real‐Time PCR test to detect bivalves including Mytilus species in foods

Sara Graziano, Mariolina Gullì, Nelson Marmiroli

International Journal of Food Science & Technology > 52 > 7 > 1567 - 1575

The incidence of allergy to seafood, and in particular to molluscs is second only to that of nuts. To protect consumers, the regulators of food products insisted on identifying molluscs as allergens. The aim was to develop quantitative assay for the presence Mytilus species in processed food products. The chosen platform was real‐time PCR (qPCR) targeting either the gene encoding mitochondrial cytochrome...

article

The global effect of exposing bakers' yeast to 5-fluoruracil and nystatin; a view to Toxichip

Sara Graziano, Mariolina Gullì, Elena Maestri, Nelson Marmiroli

Chemosphere > 2016 > 145 > C > 470-479

A genome-wide screen of a haploid deletion library of bakers' yeast (Saccharomyces cerevisiae) was conducted to document the phenotypic and transcriptional impact of exposure to each of the two pharmaceutical products 5-fluorouracil (an anti-tumor agent) and nystatin (an anti-fungal agent). The combined data set was handled by applying a systems biology perspective. A Gene Ontology analysis identified...

article

Proteoglycan-based diversification of disease outcome in head and neck cancer patients identifies NG2/CSPG4 and syndecan-2 as unique relapse and overall survival predicting factors

Anna Farnedi, Silvia Rossi, Nicoletta Bertani, Mariolina Gulli, more

BMC Cancer > 2015 > 15 > 1 > 1-19

Background Tumour relapse is recognized to be the prime fatal burden in patients affected by head and neck squamous cell carcinoma (HNSCC), but no discrete molecular trait has yet been identified to make reliable early predictions of tumour recurrence. Expression of cell surface proteoglycans (PGs) is frequently altered in carcinomas and several of them are gradually emerging as key prognostic factors...

article

Noccaea caerulescens populations adapted to grow in metalliferous and non-metalliferous soils: Ni tolerance, accumulation and expression analysis of genes involved in metal homeostasis

Giovanna Visioli, Mariolina Gullì, Nelson Marmiroli

Environmental and Experimental Botany > 2014 > 105 > Complete > 10-17

Three populations of the Brassicaceae Noccaea caerulescens: (i) the metallicolous Mt. Prinzera population (Italy), which have adapted to grow on serpentinite, a soil naturally rich in Ni, Co, Cr; (ii) the metallicolous La Calamine population (Belgium), which have adapted to growth on soil highly contaminated by Cd, Zn and Pb, and (iii) the non-metallicolous population growing near the town of Rožnov...

article

Multiplex real-time PCR assays for simultaneous detection of maize MON810 and GA21 in food samples

Maria Cristina Samson, Mariolina Gullí, Nelson Marmiroli

Food Control > 2013 > 30 > 2 > 518-525

This work describes a quantitative multiplex real-time PCR method optimized for the detection of maize MON810 and GA21. The use of specific primers and of labeled probes by real-time PCR allowed for the simultaneous detection and confirmation of amplicon identity and increased the reliability of the technique and the number of PCR applications to food analysis.Two different endogenous genes, Zein...

article

Determining resistance to Pseudomonas syringae in tomato, a comparison of different molecular markers

Ilaria Orsi, Marina Malatrasi, Enrico Belfanti, Mariolina Gullì, more

Molecular Breeding > 2012 > 30 > 2 > 967-974

Pseudomonas syringae pv. tomato (Pst) is the causal agent of bacterial speck disease in tomato. Resistance to Pst is determined by Pto, a single resistance gene that belongs to a multi-gene family clustered on chromosome 5. Pst-resistant phenotypes in cultivated tomato are determined by a semi-dominant allele of Solanum pimpinellifolium, which was introgressed into Solanum lycopersicum in the past...

article

Comparison of DNA Extraction Methods and Development of Duplex PCR and Real-Time PCR To Detect Tomato, Carrot, and Celery in Food

Simona Pafundo, Mariolina Gullì, Nelson Marmiroli

Journal of Agricultural and Food Chemistry > 2011 > 59 > 19 > 10414 - 10424

article

Quantitative detection method for Roundup Ready soybean in food using duplex real‐time PCR MGB chemistry

Maria Cristina Samson, Mariolina Gullì, Nelson Marmiroli

Journal of the Science of Food and Agriculture > 90 > 9 > 1437 - 1444

BACKGROUND: Methodologies that enable the detection of genetically modified organisms (GMOs) (authorized and non‐authorized) in food and feed strongly influence the potential for adequate updating and implementation of legislation together with labeling requirements. Quantitative polymerase chain reaction (qPCR) systems were designed to boost the sensitivity and specificity on the identification of...

article

Multiplex real-time PCR using SYBR^® GreenER™ for the detection of DNA allergens in food

Simona Pafundo, Mariolina Gullì, Nelson Marmiroli

Analytical and Bioanalytical Chemistry > 2010 > 396 > 5 > 1831-1839

We describe the development of a six-target real-time multiplex PCR assay with the SYBR® GreenER™ fluorescent dye, targeted to genes encoding for allergenic proteins commonly present in many processed food products (patent application pending). The assay was successfully trialled on reconstructed food matrices and on a range of commercial foodstuffs, and is proposed as a ready-to-use analytical tool...

article

SYBR®GreenER™ Real-Time PCR to detect almond in traces in processed food

Simona Pafundo, Mariolina Gullì, Nelson Marmiroli

Food Chemistry > 2009 > 116 > 3 > 811-815

Because food ingredients are sometimes considered as causative factors in IgE mediated food allergies, DNA-based tests may prove to be very useful to establish whether allergenic species have been used in foodstuffs production. The development of two SYBR ^® GreenER™ Real-Time PCR assays, targeting Pru 1 and rbcL genes, based on melting curve analysis, to detect allergen species in food has...

article

Methods for detection of GMOs in food and feed

Nelson Marmiroli, Elena Maestri, Mariolina Gullì, Alessio Malcevschi, more

Analytical and Bioanalytical Chemistry > 2008 > 392 > 3 > 369-384

This paper reviews aspects relevant to detection and quantification of genetically modified (GM) material within the feed/food chain. The GM crop regulatory framework at the international level is evaluated with reference to traceability and labelling. Current analytical methods for the detection, identification, and quantification of transgenic DNA in food and feed are reviewed. These methods include...

article

Four members of the HSP101 gene family are differently regulated in Triticum durum Desf.

Mariolina Gullì, Massimiliano Corradi, Patrizia Rampino, Nelson Marmiroli, more

FEBS Letters > 2007 > 581 > 25 > 4841-4849

Heat shock proteins play an essential role in preventing deleterious effects of high temperatures. In many plants, HSP101 has a central role in heat stress survival. We report the isolation and characterization of four cDNAs corresponding to different members of the durum wheat HSP101 gene family. Expression analysis revealed differences in their induction. Accordingly, durum wheat HSP101 genes are...

article

Qualitative and Quantitative Evaluation of the Genomic DNA Extracted from GMO and Non-GMO Foodstuffs with Four Different Extraction Methods

Clelia Peano, Maria Cristina Samson, Luisa Palmieri, Mariolina Gulli, more

Journal of Agricultural and Food Chemistry > 2004 > 52 > 23 > 6962 - 6968

article

Ecogeographic adaptation and genetic variation in wild barley, Application of molecular markers targeted to environmentally regulated genes

Lucia Liviero, Elena Maestri, Mariolina Gulli, Eviatar Nevo, more

Genetic Resources and Crop Evolution > 2002 > 49 > 2 > 133-144

Long primer PCR (LP-PCR) markers specifically targeted to sequences involved in the response to abiotic stress were utilised to analyse genetic variation within and among wild barley populations from Israel.Populations were sampled in locations reflecting a wide range of habitats and the potential correlation between genetic variation and ecogeographic parameters was examined. A high level of polymorphism...

article

Molecular genetics of heat tolerance and heat shock proteins in cereals

Elena Maestri, Natalya Klueva, Carla Perrotta, Mariolina Gulli, more

Plant Molecular Biology > 2002 > 48 > 5-6 > 667-681

Heat stress is common in most cereal-growing areas of the world. In this paper, we summarize the current knowledge on the molecular and genetic basis of thermotolerance in vegetative and reproductive tissues of cereals. Significance of heat stress response and expression of heat shock proteins (HSPs) in thermotolerance of cereal yield and quality is discussed. Major avenues for increasing thermotolerance...

article

Characterization of two barley genes that respond rapidly to dehydration stress

Maria Grossi, Mariolina Gulli, A. Michele Stanca, Luigi Cattivelli

Plant Science > 1995 > 105 > 1 > 71-80

The present study investigates the barley cDNA clones paf93 and cdr29 which correspond to mRNAs regulated by low temperature and drought stress but not by exogenous ABA treatment. During the early stage of dehydration, these mRNAs are expressed before the induction of known ABA regulated genes such as dehydrins and when only a small increase occurs in ABA content. The cDNA clone cdr29 cross-hybridizes...

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