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Abstract. To assess protection of the mesophyll cell plasmalemma against O3 by apoplasmic reduced ascorbate (AA), its concentration in the leaf cell wall of common bean (Phaseolus vulgaris L.) was lowered from 0.6mM to 0.1mM by pre-exposing plants to continuous darkness for up to 48h. Subsequent ozonization of ascorbate-deficient leaves with 350450nmol O3mol1 resulted in a rapid rise of apoplasmic...