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The sps1 gene, encoding the sucrose phosphate synthase (SPS) in rice has an unusually long 5' untranslated region (leader) of 368 nt with a complex predicted secondary structure. Elimination of the leader from the native sps1 promoter diminishes the in vivo expression of the GUS reporter gene by 10-fold. Insertion of the leader downstream, but not upstream, of the -46(35S) CaMV promoter in the correct...
Genomic and cDNA clones coding for elongation factor-1β (eEF-1β) from Arabidopsis thaliana (At) were isolated and characterized. eEF-1β was found to be encoded by a single-copy At gene. Chimeric genes fusing the promoter and the 5 untranslated region of the At eEF-1β gene to the gus reporter gene were constructed and used to study the expression of this gene in transgenic tobacco plants. Interestingly,...
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