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Sucrose phosphate synthase (SPS) is a key enzyme in the translocation of photoassimilates from sink-to-source tissues and the remobilization of reserves from storage tissues during seed germination. The tissue specific distribution of SPS is mainly determined by transcriptional mechanisms. To gain further insight into the mechanisms that regulate the transcriptional activity of SPS genes, we carried...
The sps1 gene, encoding the sucrose phosphate synthase (SPS) in rice has an unusually long 5' untranslated region (leader) of 368 nt with a complex predicted secondary structure. Elimination of the leader from the native sps1 promoter diminishes the in vivo expression of the GUS reporter gene by 10-fold. Insertion of the leader downstream, but not upstream, of the -46(35S) CaMV promoter in the correct...
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