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We applied nonlinear optimization to convert a cannula into a high-resolution computational-fluorescence microscope. The microscope was used to image fluorescent microspheres, genetically-encoded mouse-brain slices, genetically-encoded and fluorescent-stained C. elegans nematodes (both living and dead animals).
Fluorescent miscroscopy is a state-of-the-art method for creating high contrast and high resolution images of microscopic structures and has found wide application in microendoscopy (i.e., imaging cellular information from an optical probe within an animal). Cannula based microscopy methods have recently shown great promise for efficient microendoscopy imaging. Yet, performing real-time imaging with...
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