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We synthesized a novel multi-fluorescence sensor which can respond to both pH and temperature. This kind of sensor was based on amino-polystyrene microbeads and two different fluorescent indicators (Rhodamine B and FITC) were applied simultaneously to synthesize the multi-sensor. Rhodamine B shows an excellent linear relationship between the relative fluorescent intensity and temperature while it...
Using optical tweezers for trapping and infection of influenza virus influenza virus binds selectively G1 phase cell. This result encouraged us to dissolve the difference between G1- and S/G2/M-phage cells. At the beginning, we tried to analyze the sialic acid content of both G1-and S/G2/M-phase cells. Because it had been shown that influenza virus attached to the sialic acid on the cell membrane...
In this research, we demonstrated a microfluidic chip to pretreat the samples for viral genome assay. The microfluidic chip has the following three functions; (1) Virus purification and enrichment, (2) Viral RNA extraction, and (3) Capture of the targeted virus genome. Hydroxyapatite chromatography, Boom method, and PNA (Peptide Nucleic Acid) were used for the above three functions, respectively....
We have succeeded in protein crystallization and processing by electrically-induced bubble knife. The dispensed directional mono-dispersed micro-bubbles whose air-liquid interface tends to combine the ambient molecules due to by their stiction force on the surface of micro-bubbles. Electrically-induced bubble knife enables to generate mono-dispersed micro-bubbles by the electrolysis and local heat,...
We present an innovative gel actuator made of photo-processible thermoresponsible gel. (Fig. 1) We evaluated integratability of the gel actuator by experimentally and theoretically and confirmed that it is possible to integrate within 100 μm. We performed demonstration of integrated valves fabricated on five branched microchannel.
We proposed hybrid nanorobot that can be manipulated by optical tweezers and has hybrid structure integrating functional nanomaterials. We employed femtosecond laser exposure for fabrication of the nanorobot having 3D structure with nanometric size and integrating functional nanomaterials. We succeeded in fabricating nanorobot with carbon nanotubes (CNTs) that has a high photothermal efficiency. We...
3D tailor-made biodegradable scaffold integrated with biological cells or molecules is of great importance for tissue engineering. This paper addresses an improved method for exploring magnetic tweezers in patterning and aligning magnetic hydrogel fiber to fabricate large-scale engineered cell-hydrogel constructs. Magnetic hydrogel fibers were fabricated based on microfluidic device. The fabricated...
This paper presents a novel measurement system of cellular mechanical properties based on a robot integrated microfluidic chip. In order to achieve the high throughput measurement of cellular mechacal properties, we proposed the robot integrated microfluidic chip (robochip), taking advantages of both of micromechanical manipulator and Lab-on-a-Chip devices. The robochip contained a pair of a magnetically...
The CTCs (Circulating Tumor Cells) isolation method enables to sort very rare single CTC from the whole blood. We utilized the technique of convective self-assembly, which is a technique for depositing cells onto the microfluidic channel, to isolate CTCs. Through the convective self-assembly processes, the cells including CTCs and blood cells are deposited in the channels of microfluidic chip. After...
Recently, the number of circulating tumor cells (CTC) in peripheral blood from tumor patients have been recognized as an important biomarker for patient prognosis, especially in breast and prostate cancer. Although CTC has potential as a “liquid biopsy,” it is too small in number to apply for proteomics and genetic analysis. Several devices are currently provided, but are still insufficient. Therefore,...
This paper reports a novel on-chip cellular force measurement method of single cells using Direct-Outer-Drive mechanism (D-O-D mechanism). This D-O-D mechanism enables high speed (1 kHz), high power (1 kN) and high accurate manipulation (nanometer-order) of Robochip (Robot-integrated microfluidic chip) and mechanical property measurement of single cells. We succeeded in mechanical property measurement...
In this paper, we discuss a novel, magnetically driven microrobot equipped with a frame structure to measure the stimulant property of aquatic microorganisms. The fabricated microfluidic chip and microrobots are shown. The untethered microrobot is composed of a Si-Ni hybrid structure constructed by MEMS techniques. The microrobots with 5 μm width force sensors are actuated in a microfluidic chip by...
Bio-actuated, especially flagellated bacteria-driven, microrobots have been actively studied to develop novel technologies such as microsurgery inside human body, though the characteristics of the bacterial flagellar motor have not been sufficiently investigated. For analysis of the flagellar motor, we previously proposed the local environmental control system with nano/micro dual pipettes to change...
This paper reports fabrication of retinal microvessels model for evaluation of microcannulation. Because microcannulation as a new treatment for retinal vein occlusion is difficult surgery operation, development and evaluation of microsurgical robot systems to assistant vitreoretinal surgery is needed. Evaluation of microcannulation needs a retinal microvessels model without individual difference...
High sensitive, wide-measurement range, and small sized load sensors was developed by using AT-cut quartz crystal resonator (QCR). The quartz crystal generates a charge which is proportional to the external force. Since it has high sensitivity and excellent temperature stability, it has been used for various sensors. Especially, QCR has superior characteristic for static load sensing in nature. However,...
A method for simultaneously tracking multiple cells in a microchannel is proposed in this paper. The instantaneous size, position and velocity of cell though a microchannel can be obtained by using the method. In order to improve both the success rate and computing speed of the tracking, two possible fault cases are classified, and the fault cases are avoided during the image process. Red blood cells...
BackgroundQuantification of medical skills is a challenge, particularly simulator‐based training. In the case of endovascular intervention, it is desirable that a simulator accurately recreates the morphology and mechanical characteristics of the vasculature while enabling scoring.
MethodsFor this purpose, we propose a cyber‐physical system composed of optical sensors for a catheter's body motion...
A method for improving the evaluation of cell deformability is proposed. Cell deformability can be evaluated by the ratio between the cell velocities before and after entering the narrow throat of a microchannel. However, the flow-in velocity, the cell velocity before entering the throat, varies with respect to the location of the cell because of the laminar flow inside the microchannel. To compensate...
We proposed a 3D assembly technique using water transfer printing to fabricate a multilayer structured tubular tissue. This study was aimed to determine whether the tissue-engineered tubular structure maintains the normally mechanical property as the development and function by the artificial circulatory system. In this work, we demonstrated that fabricated tissues could rapidly assemble into aligned...
In this paper, we developed transfection of a single fluorescent nanosensor into a cell nucleus using step-wise fusion of liposome for cellular temperature measurement. The single fluorescent temperature nanosensor is included in the multi lamellar liposomes using Layer-by-Layer formation. The sensor is manipulated by optical tweezers and transfected into the cell or cell nucleus by membrane fusion...
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