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We developed selective and rapid injection of fluorescence sensor into the cell for intracellular measurement. The sensor encapsulated in the liposome contains photochromic chemical, 1, 3, 3-Trimethylindolino-6'- nitrobenzopyrylospiran, for optical control of zeta potential. The molecular structure of spiropyran changes between spiropyran type and merocyanine type by UV/VIS illumination. Zeta potential...
Multi-fluorescent sensor has been synthetized using two different fluorescent indicators (Rhodamine B and FITC) which can respond to both pH and temperature. A method of selective adhesion and rapid injection of the sensor into the individual cell have also been proposed. The sensor was encapsulated in the liposome layers containing photochromic material, 1, 3, 3-Trimethylindolino-6'- nitrobenzopyrylospiran...
We developed selective transfection of single fluorescence sensor into a specific cell by optically controlled cell immobilization and membrane fusion of lipid capsule for intracellular measurement. The single fluorescent sensor was encapsulated in the lipid capsule using novel packaging based on a multi-layering method. The liposomes with number-controlled lipid bulyer contain 1,3,3-Trimethylindolino-6'-nitrobenzopyrylospiran...
We developed selective injection of a single particle sensor into a specific cell by optically-regulated cell adhesion and membrane fusion of liposome for intracellular measurement. Fluorescence sensor encapsulated in fusogenic liposome is fabricated by spontaneous transfer method. The liposome is consists of neural lipids and spiropyran (SP). SP is a photochromic chemical and used to control the...
We developed Optical pH regulation using functional nanotool impregnated with photo-responsive chemical for selective cell injection of nanotool. The nanotool was modified by fluorescent dye for intracellular measurement. The nanotool was included in the fusogenic liposome. Membrane fusion of the liposome to the cell membrane was used for invasive cell injection of the nanotool. The liposome fuses...
We developed Optical pH regulation using functional nanotool impregnated with photo-responsive chemical for selective cell injection of nanotool. The nanotool was modified by fluorescent dye for intracellular measurement. The nanotool was included in the fusogenic liposome. Membrane fusion of the liposome to the cell membrane was used for invasive cell injection of the nanotool. The liposome fuses...
We developed selective cell injection of nanotool using photo responsive chemical for intracellular measurement. The nanotool was included in the fusogenic liposome. Membrane fusion of the liposome to the cell membrane was used for invasive cell injection of the nanotool. The liposome fuses to the cell in weak acidic condition. Local pH control inside the liposome was developed by using photo responsive...
An on-demand adhesive control of functional gel-tool for on-chip cell experiment was developed. Adhesiveness of the gel-tool was controlled by UV illumination in an electrolyte solution of an adjusted electrolyte concentration. Gel-tool adheres to objects such as glass substrate, other gel-tool and cell by contact to the target object under UV illumination. Without UV illumination, gel-tool does not...
A novel multiple environments measurement with functional gel-microbead was developed. Optical multiple microsensor was fabricated by connecting gel-microbeads impregnated different indicators. In this paper, Bromothymol blue (BTB) and Bromocresol green (BCG) were employed as pH indicators. BTB and BCG have different indicator range. Rhodamine B is temperature sensitive fluorescent dye and is used...
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