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Immunosensor is an ideal kind of biosensor, which enables highly specific detection of any kind of biological molecules. However, in spite of long and intensive efforts of research and development, no immunosensor is in practical use. This is mainly because of the difficulty in reduction of the back ground noise of non-specific adsorption. In this study, fluorescence anisotropy decay principle was...
In this study, high separation ratio microfluidic cell sorting by a dielectrophoretic (DEP) force is presented. The recent development of iPS cell technology has raised the possibility of regenerative therapy. Technologies of genetic cell processing require purification of specific target cells by a cell sorter. The most widely used system for cell separation is flow cytometry (FCM), which has been...
The blood test is a routine procedure in medical diagnosis. However it takes a couple of days to get the results because blood samples are analyzed by sending to the specialized facility because the operation of the blood test instrument is costly for a hospital or clinic. So development of a novel low cost blood test device is an important issue considering the burden of the patient. To developing...
Fluorescence anisotropy of GFP molecules, suspended in solution and immobilized on a glass substrate, was measured to investigate the application for nano-environment probe. Nano-environment around living cell is of interest in relation with wide range of fields, such as cell interaction in cancer tissue, nerve signal transmission and so on. However, nano-environment is so sensitive to the interference...
The purpose of this study is the development of a medical cell sorter. Cell sorters that are currently used were developed for research, but they have problems of high running cost or risk of infection in medicine. Here, we reduce operating costs by using a photolithography technique. This cell sorter uses dielectrophoretic force to act on non-charged particles in a non-uniform electric field, and...
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