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This paper proposes an automatic measurement algorithm for calculating statistics of pulp fiber lengths and curvature. The fibers are extracted from digital images, and a parametric model is fit to the extracted objects. The modeling approach improves the robustness of the detection and enables connecting broken fiber parts into full length curves. The developed algorithm is compared to an alternative...
This article presents a synthetic image set for validation of cell image analysis algorithms. To address the problem of validation, we have previously developed a simulation framework for cell population images. Here, we apply the simulation for generating a benchmark set of cell images with varying characteristics. The value of simulation is in the ground truth information known for the generated...
An automated image analysis method for extracting the number of peroxisomes in yeast cells is presented. Two images of the cell population are required for the method: a bright field microscope image from which the yeast cells are detected and the respective fluorescent image from which the number of peroxisomes in each cell is found. The segmentation of the cells is based on clustering the local...
An automated image analysis method for extracting the number of peroxisomes in yeast cells is presented. Two images of the cell population are required for the method: a bright field microscope image from which the yeast cells are detected and the respective fluorescent image from which the number of peroxisomes in each cell is found. The segmentation of the cells is based on clustering the local...
Detection and three dimensional reconstruction of cell structures from brightfield microscopy video clips using digital image processing algorithms is presented. While the confocal microscopy offers an efficient technique for three dimensional measurements, extensive and repeated measurements are still often better to be performed using permanent staining and brightfield microscopy. By processing...
Detection and three dimensional reconstruction of cell structures from brightfield microscopy video clips using digital image processing algorithms is presented. While the confocal microscopy offers an efficient technique for three dimensional measurements, extensive and repeated measurements are still often better to be performed using permanent staining and brightfield microscopy. By processing...
A new automated image analysis method for quantification of fluorescent dots is presented. This method facilitates counting the number of fluorescent puncta in specific locations of individual cells and also enables estimation of the number of cells by detecting the labeled nuclei. The method is here used for counting the AM1-43 labeled fluorescent puncta in human SH-SY5Y neuroblastoma cells induced...
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