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Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is usually applied to small-scale basic research studies of complex genetic diseases that are associated with single nucleotide polymorphisms (SNPs). Before performing PCR-RFLP for SNP genotyping, the feasible primer pair and the available restriction enzymes for discriminating the target SNP are required. This is a tedious...
The polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique is often used in laboratories and many basic research studies of complex genetic diseases associated with single nucleotide polymorphisms (SNP). When performing PCR-RFLP for SNP genotyping, feasible primer pairs are subject to numerous constraints and require a restriction enzyme for discriminating the target...
Designing a feasible primer pair is an important work before performing polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for single nucleotide polymorphism (SNP) genotyping. However, in many cases, no restriction enzymes are available to discriminate the target SNP, thus rendering the primer design useless. We propose a method that uses a genetic algorithm (GA) to search...
Many single nucleotide polymorphisms (SNPs) genotyping techniques have been developed but most of them are expensive. Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) is a restriction enzyme-free and economic genotyping but its primer design is still computationally challenged. Here, we introduced a genetic algorithm (GA)-based PCR-CTPP primer design method. Thirty SNPs of the...
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