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The majority of Escherichia coli O157:H7 outbreaks are associated with ground beef. To detect this pathogen, separation techniques were tested with E. coli O157:H7 in ground beef followed by FT-IR analyses. Ground beef samples were inoculated with various levels of live and heat treated E. coli O157:H7 cells and the bacteria were extracted by filtration or immunomagnetic separation (IMS). Spectra...
Fourier-transform infrared spectroscopy (FT-IR) utilizes nanostructural differences between bacterial cells for the rapid identification, classification, and differentiation of many species of bacteria, but most studies have used only live cells. A rapid and reliable method for determining the total number of live and dead bacterial cells in a food could prevent the distribution of unsafe products...
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