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In order to study the effect of butyl benzyl phthalatee (BBP) on hepatic cells of rat, hepatic cells of rat were exposed to different dose (5 μmol / L, 20 μmol / L, 80 μmol / L) of BBP in vitro for 1 hour. Thiobarbituric acid (TBA) method , KCl-SDS precipitation method and Dinitrophenylhydrazone (DNPH) colorimetric method were used to detect malondialdehyde (MDA) content, DPC coefficient changes and...
The effect of Di-(2-ethylhexyl) Phthalate was investigated when alga Scenedesmus obliquus has been exposed to different concentrations (5, 10, 20, 40 and 80 mg · L-1) at 24, 48, 72 and 96 hours of exposure. Toxicity test was done by using four biomarkers including growth inhibition rate and the content of chlorophyll a, chlorophyll b and carotenoid. This study indicated that with the increase concentration...
We developed a quantitative detection method that can reflect the degree of visceral cell oxidative DNA damage induced by exogenous compound exposures using 8-OHdG as a biomarker in vitro. In this study formaldehyde (FA) was used as a model exogenous compound and malondialdehyde (MDA) was used as a reference indicator. Rat liver cell suspension was applied in this in vitro exposure experiments as...
To study the effect of Butylbenzyl phthalate inducing DNA damages in Rat lung cells. KCl-SDS assay and comet assay were used to reveal the amount of DNA-protein crosslinks and effect of DNA damages induced by different doses of Butylbenzyl phathalate in vitro. The results showed that Butylbenzyl phathalate could cause DNA breakage at low level (10 μmol/L, P<;0.01), while at higher level (40 μmol/L,...
Owing to its good flame-retardant effect, polybrominated diphenyl ethers (PBDEs) were widely added to electronic, electrical appliances, as well as textiles and chemical products. Decabromodiphenyl Ether (BDE-209) is one class of PBDEs which was most demanded in international market. To illustrate the possible function of BDE-209 on rat liver, hepatic cells were expose to BDE-209 with different concentrations...
In this study, the gene encoding purine nucleoside phosphorylase (PNPase) from Bacillus subtilis W168 was identified, cloned and expressed in Bacillus subtilis AG208. The gene encodes a polypeptide of 233 amino acids with a calculated molecular weight of 25,018 Da. The enzyme activity of the recombinant protein (AGPNP) was analyzed by temperature and pH perturbation difference spectra. Results showed...
Accumulating evidences demonstrate that the channeling of nitric oxide (NO) into S-nitrothiols (SNOs) plays a pivotal role in mediating NO actions. Recent work suggests that GSNO reductase (GSNOR), an enzyme responsible for SNO metabolism, is abnormally elevated under asthmatic conditions. To study GSNOR induction in mice lung, male Kun Ming mice were exposed to gaseous formaldehyde (FA) of 0, 3.0...
How does inhaled formaldehyde transfer the genotoxicity from exposure organ to site distant organs via blood? This is the key question to understand leukemia induced by formaldehyde. To answer this question, we used new born calf serum (NBS) as "blood" and Hela cells as materials to investigate its effects on cellular thiol concentration and DNA-protein crosslink (DPC) induced by formaldehyde...
The objective of this study is to explore the relation between oxidative stress and DNA-protein crosslink formation caused by formaldehyde (FA) inhalation in mice liver. 15 Kun Ming mice were divided into 3 groups: control group, 3.0 mg/m3 gaseous FA inhaled group, 3.0 mg/m3 gaseous FA and lipoic acid (LA) treated group. The content of reduced glutathione (GSH) and the content of DPC were measured...
In the past decade, rapid development happened in the field of nanotechnology. Recently, nano-material, especially inorganic hybrid molecule, has become an increasingly hot issue in medicine area for safe drug delivery system. In this paper, nano-layered double hydroxides (nano-LDHs) were chosen to be a potential drug carrier. In order to explore the biocompatibility of nano-LDHs with Hela cells,...
The purpose of the present study was to examine levels of mRNA for DNA methyltransferases (DNMTS) and methyl- CpG-binding proteins 2 (MeCP2 ) in the lung of mouse after middle-level gaseous formaldehyde exposure. Male mice were exposed to clean air or 3.0 mg/m3 formaldehyde for consecutive 72 h and the lungs were dissected for RNA extraction. The mRNA levels of dnmtl, tin in t3a and mecp2 gene were...
Recent work showed that GSNO reductase (GSNOR) mediates the equilibrium of nitric oxide (NO) and the adduct molecules, S-nitrosothiols (SNOs) in lungs of asthmatic mice, and thus, plays a central role in the physiology and patho-physiology in lung diseases. To further understand the role of GSNOR in mediating asthma development, it is essential to examine the possible factors that can activate GSNOR...
Objective: To explore the DNA damage of brain cells of rat after formaldehyde exposure. Methods: Rat brain cells were exposed to different concentrations of liquid formaldehyde (0.005, 0.025, 0.125 and 0.625 mmol/L) for lh. Single cell gel electrophoresis technique (comet assay) was used to test the DNA damage of the brain cells. Result: Formaldehyde could significantly cause DNA strands breakage...
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