In order to study the effect of butyl benzyl phthalatee (BBP) on hepatic cells of rat, hepatic cells of rat were exposed to different dose (5 μmol / L, 20 μmol / L, 80 μmol / L) of BBP in vitro for 1 hour. Thiobarbituric acid (TBA) method , KCl-SDS precipitation method and Dinitrophenylhydrazone (DNPH) colorimetric method were used to detect malondialdehyde (MDA) content, DPC coefficient changes and content, respectively. MDA levels in hepatic cells showed a dose-dependent enhancement after BBP exposure. Significant MDA content was observed in all groups compared to control group. The content of carbonyl was all increased in exposure groups, especially in the group of 5 μmol / L. DPC coefficient presented a slight increase compared with the control group but no significant difference in the dose of 5 μmol / L and 20 μmol / L. When the concentration of BBP increased to 80 μmol / L, DPC coefficient increased, and there was a significant difference compared with the control group. The carbonyl content was significantly increased in the dose of 5 μmol / L. These results suggest that BBP can cause liver damage in rats, and might have toxic effects on the human body.