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Ceratoides lanata is the native sand-fixing plant in deserts of America Continent. As an even-green undershrub with extremely strong antifreeze capability, Ceratoides lanata had been considered as the winterfat and introduced into many countries including China. To explore the mechanism of cold resistance and to clone genes directly involving the strong antifreeze capability, a normalized cold-stressed...
The transformation of cDNA into plant is still not carried out directly by a universal transgenic vector, hence it is not convenient for the transgenic application of those function-important cDNAs. Herein we introduced a rapid, cost-effective PCR-based DNA synthesis method to construct a new universal plant overexpression vector for the direct inserting of target cDNA during transgenic application...
Antifreeze protein (AFP) can help organisms survive from freezing condition by thermal hysteresis activity to control ice growth and by recrystallization inhibition activity to inhibit recrystallization of ice granules, respectively. By bioinformatics methods especially protein docking, the newly developed models of surface complementarity were used to obtain the molecular models of AFPs and hence...
The branchlet and nutshell of Camelliaoleifera are produced in quantity while picking and processing the nut each year. However, the abundant branchlet and nutshell of C.oleifera are still underutilized and hence wasted to pollute environment. Therefore, GC/MS technology was used to analyze the top value-added components from the branchlet and nutshell of C.oleifera. After treated with freeze-drying,...
Wood extractive brings more difficulties on the treatment of polluting wastewater from wood industry and paper mill, Therefore, many enterprises have to raise pretreated procedure of wood chips of papermaking to decrease the disadvantageous impacts from wood extractive. However, wastewater from wood chip pretreatment becomes a difficult and urgent issue. Eucalyptus camaldulensis has been widely planted...
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